Jackie L. Collier
Ph.D., 1994, Stanford University
Phytoplankton physiological ecology; Biocomplexity and microbial diversity; Planktonic ecosystem processes in marine, estuarine, and freshwater systems
The long-term goal of my research program is to improve our understanding of phytoplankton physiological ecology, population dynamics, community structure, and ecosystem roles by taking an autecological approach to investigating the lives of these microorganisms. The tools we use to answer these questions include observational and experimental (e.g., dilution gradient and nutrient addition) field studies, which are analyzed by techniques such as flow cytometry; lab-based investigations of phytoplankton physiology using various isolates growing in culture; and a variety of molecular biological, molecular genetic, and biochemical techniques. For many reasons, the cyanobacteria are the predominant model system used in my lab.
One part of my lab is focused on investigating basic cyanobacterial molecular genetics and physiology. For example, with support from DOE we are investigating the function of a thioredoxin-like gene, TxlA, which is found only in cyanobacteria and photosynthetic eukaryotes. Also with support from DOE and in collaboration with Chip Lawrence and coworkers at Wadsworth, we are just embarking on a new project that will take advantage of the availability of several complete cyanobacterial genome sequences to define the transcription regulation networks in cyanobacteria.
Another major focus of work in my lab grew out of my interest in the utilization of urea as a nitrogen source by marine Synechococcus. As part of an NSF-funded Biocomplexity project (http://geoweb.princeton.edu/research/biocomplexity/index.html), we are investigating the biochemically-defined functional group of microorganisms that can degrade urea, which is one of many potentially important but poorly understood forms of organic nitrogen present in aquatic ecosystems. Since most organisms use the well-conserved enzyme urease to degrade urea, we have designed oligonucleotide primers that are expected to be universal; that is, they should enable us to amplify any urease gene. Application of these primers to samples from Chesapeake Bay has revealed a very high diversity of urease sequences. Our current efforts are focused on developing a similar approach to describe the diversity of phytoplankton, and to adapt both to high-throughput technologies, such as gene array hybridization.
A third part of my lab is focused on investigating the comparative ecology of the small (<2 mm) planktonic picocyanobacteria that are found in both marine and freshwater ecosystems. We have been using flow cytometry and molecular techniques to investigate the picocyanobacteria in Lake George, NY, which we have found to be numerically dominated by organisms very much like marine Synechococcus. A similar project, funded by the Hudson River Foundation, is underway in the Hudson River Estuary, where we are seeking to define the role of picophytoplankton in the estuarine food web.
Waller R.F., Cleves P.A., Rubio-Brotons M., Woods A., Bender S.J., Edgcomb V., Gann, E.R., Jones, A.C., Teytelman, L., von Dassow, P., Wilhelm, S.W., and Collier, J.L. (2018) Strength in numbers: Collaborative science for new experimental model systems. PLoS Biol 16(7): e2006333. https://doi.org/10.1371/journal.pbio.2006333
Mao, X., Waugh, S., Langlois, K., Collier, J.L., Price, R., Garvey, J., Gobler, C. and Walker, H., 2017. Nitrogen transformations and microbial characterization in passive nitrogen removing biofilters (NRBs) for onsite wastewater treatment. Proceedings of the Water Environment Federation, 2017(15), pp.898-906.
P. J. Keeling, F. Burki, H. M. Wilcox, B. Allam, E. E. Allen, L. A. Amaral-Zettler, E. V. Armbrust, J. M. Archibald, A. K. Bharti, C. J. Bell, B. Beszteri, K. D. Bidle, C. T. Cameron, L. Campbell, D. A. Caron, R. A. Cattolico, J. L. Collier, K. Coyne, S. K. Davy, P. Deschamps, S. T. Dyhrman, B. Edvardsen, R. D. Gates, C. J. Gobler, S. J. Greenwood, S. M. Guida, J. L. Jacobi, K. S. Jakobsen, E. R. James, B. Jenkins, U. John, M. D. Johnson, A. R. Juhl, A. Kamp, L. A. Katz, R. Kiene, A. Kudryavtsev, B. S. Leander, S. Lin, C. Lovejoy, D. Lynn, A. Marchetti, G. McManus, A. M. Nedelcu, S. Menden-Deuer, C. Miceli, T. Mock, M. Montresor, M. A. Moran, S. Murray, G. Nadathur, S. Nagai, P. B. Ngam, B. Palenik, J. Pawlowski, G. Petroni, G. Piganeau, M. C. Posewitz, K. Rengefors, G. Romano, M. E. Rumpho, T. Rynearson, K. B. Schilling, D. C. Schroeder,A. G. B. Simpson, C. H. Slamovits, D. R. Smith, G. J. Smith, S. R. Smith, H. M. Sosik, P. Stief, E. Theriot, S. N. Twary, P. E. Umale, D. Vaulot, B. Wawrik, G. L. Wheeler, W. H. Wilson, Y. Xu, A. Zingone, A. Z. Worden. 2014. The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): Illuminating the Functional Diversity of Eukaryotic Life in the Oceans through Transcriptome Sequencing. PLoS Biology 12(6):e1001889. DOI: 10.1371/journal.pbio.1001889
Koch, F., Burson, A., Tang, Y. Z., Collier, J. L., Fisher, N. S., SaÃ±udo-Wilhelmy, S., & Gobler, C. J. (2014). Alteration of plankton communities and biogeochemical cycles by harmful Cochlodinium polykrikoides (Dinophyceae) blooms. Harmful Algae, 33(0), 41-54. doi: http://dx.doi.org/10.1016/j.hal.2014.01.003
Zani, S., M.T. Mellon, J.L. Collier, and J.P. Zehr. 2000. Expression of nifH genes in natural microbial assemblages in Lake George, NY detected with RT-PCR. Applied and Environmental Microbiology 66: 3119-3124.
Collier, J.L. 2000. Flow cytometry and the single cell in phycology. Journal of Phycology 36: 628-644.
Collier, J.L. and L. Campbell. 1999. Flow cytometry in molecular aquatic ecology. In: Molecular Ecology of Aquatic Ecosystems. J.P. Zehr, ed. Kluwer Academic Publishers. Dordrecht, The Netherlands. Hydrobiologia 401:33-53.
Collier, J.L., B. Brahamsha, and B. Palenik. 1999. The marine cyanobacterium Synechococcus sp.WH7805 requires urease (urea amidohydrolase, EC 126.96.36.199) to utilize urea as a nitrogen source: molecular genetic and biochemical analysis of the enzyme. Microbiology UK 145:447-459.
Collier, J.L., and A.R. Grossman. 1995. Disruption of a novel thioredoxin-like protein alters the cyanobacterial photosynthetic apparatus. Journal of Bacteriology 177:3269-3276.
Apt, K.E., J.L. Collier, and A.R. Grossman. 1995. The evolution of the phycobiliproteins. Journal of Molecular Biology 248:79-96.
Collier, J.L., S.K. Herbert, D.C. Fork, and A.R. Grossman. 1994. Changes in the cyanobacterial photosynthetic apparatus during acclimation to macronutrient deprivation. Photosynthesis Research 42:173-183.
Bhalerao, R.P., J.L. Collier, P. Gustaffson, and A.R. Grossman. 1994. The structure of phycobilisomes in mutants of Synechococcus sp. Strain PCC 7942 devoid of specific linker polypeptides. Photochemistry and Photobiology 61:298-302.
Collier, J.L. and A.R. Grossman. 1994. A small polypeptide triggers complete degradation of light-harvesting phycobiliproteins in nutrient-deprived cyanobacteria. EMBO (The European Molecular Biology Organization Journal) 13:1039-1047.
Collier, J.L. and A.R. Grossman. 1992. Chlorosis induced by nutrient deprivation in Synechococcus sp. strain PCC 7942: Not all bleaching is the same. Journal of Bacteriology 174:4718-4726.
From Claire Garfield ’20 Awarded Prestigious Marshall Scholarship by Robert Emproto on Stony Brook News, December 9, 2019 Claire Garfield ’20 has been awarded the Marshall Scholarship, one of the most prestigious academic honors available to U.S. undergraduates. The...
Photo above: Dean Shepson welcomes the Class of 2019 at the SoMAS Convocation. Congratulations to our graduates! The annual SoMAS Convocation occurred on Friday, May 24, 2019 at the Center for Leadership and Service. Students gathered with their friends and family...
Souper Bowl V proved more than a match for the icy weather. Thanks to the cooks for providing blasts of delicious warmth to beat back the arctic chill: Betsy Barrows - Chicken Chili Jackie Collier - Is It Vegan? Chili Maureen Murphy - Chocolate Cake and Irish Soda...
Photo above, from left: Dr. Darcy Lonsdale with Graduate Liblit Scholar Karin Schweitzer and Michael Cahill, Chair of the Liblit Scholarship Steering Committee The 21st annual Evan R. Liblit Scholarship/Fundraiser Breakfast took place on November 14 at the...
From Researchers of the Month November 2018 at URECA This month, URECA features two students who have been active in research in the School for Marine & Atmospheric Sciences (SoMAS). Both currently participate in the Stony Brook Southampton: Semester by the Sea...
SoMAS students and faculty have spent some of their summer preparing for conferences, giving talks and training future scholars. Here are a few updates: Nicholas Leonardo, Ph.D. candidate in Prof. Brian Colle's group, was awarded Best Student Oral...
Photo above: Marine protists known as thraustochytrids produce carotenoids, which are responsible for giving them a yellow to orange color. Carotenoids are essential nutrients for animals, and of biotechnological interest for several applications. EMS scientists are...
The School of Marine and Atmospheric Sciences at Stony Brook University hosted the 2018 Bay Scallop Bowl on February 3rd, 2018 at the Student Activities Center. This year, twelve teams competed for a chance to move on to the National Ocean Sciences Bowl...
SoMAS Faculty and staff were among the honorees at the Fall University Awards Dinner where they were recognized for their hard work and dedicated service by Interim Provost and Senior Vice President for Academic Affairs Charles Taber. Congratulations to them all!...
SoMAS faculty and alumni attending the 2016 Ocean Sciences Meeting in New Orleans gathered for an alumni reunion. Professors Gordon Taylor and Mary Scranton organized the event and invited SoMAS alumni present at the conference to reunite. Other SoMAS faculty in...